Lab Chip. 2020 Apr 25. Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract. Sun F1, Ganguli A2, Nguyen J3, Brisbin R4, Shanmugam K4, Hirschberg DL5, Wheeler MB6, Bashir R7, Nash DM8, Cunningham BT7. Author information 1 Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu. 2 Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA. 3 RAIN Incubator, Tacoma, Washington, USA. 4 Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA. 5 RAIN Incubator, Tacoma, Washington, USA and Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA. 6 Department of Animal Sciences, University of Illinois at Urbana-Champaign, Illinois, USA. 7 Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu and Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA. 8 Private equine veterinarian, Kentucky, USA. Abstract Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
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